FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e2-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e2-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294162
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACCC6101.1001684E-68.60750347
ATTAACG4500.0020131017.77712971
TGTCATG16050.07.63176312
TTCTAGG4600.00249707147.6083558
GATGTCA13950.07.526544610
TGATGTC13751.8189894E-127.3814889
CCCGATC6653.2119067E-57.36894651
GTCATGT15900.07.263545513
CCGATCT7351.5556023E-57.143642452
ATATCGA6451.7630369E-47.05367571
GCCTTTA7451.8802708E-57.04775448
CGCAACC11059.3605195E-96.969091446
CAACCCG6107.1660406E-46.886002548
CTTGATG17350.06.8584837
ATCCTTG13005.2623363E-96.46099951
GAGTATA21200.06.43789428
CTTTATG9309.060041E-66.3973161
CTATATT12702.2107997E-86.33805471
TGAGTAT22650.06.334768327
TCATGTG19550.06.2652214
Produced by FastQC (version 0.11.5)