FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e2-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e2-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724016
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCT2300.00788470710.65203811
CCCAATT3150.00779349458.8887754
ATCCTGA3150.00779790948.8881611
ATATACA11951.8189894E-127.90730241
CTCTTAT5855.2549563E-57.7771411
ATATACT8803.818697E-66.760811
ATATGCA5700.00266010036.7538331
TAGGCTT7004.7760553E-46.500365745
CTTTAAG7257.3198223E-46.27534771
ATATGAT13409.998985E-96.26814371
TATACTC11852.5664485E-76.2024527
CTTATAC11357.799081E-76.16689631
TTGCGGT7500.00109965636.067007563
ATAATAG7050.0036671475.9569591
TGTGTCT8952.3754544E-45.86503718
TTGGCTT9609.770017E-55.833258611
ATATGAG11405.4907196E-65.83285571
ATATTGA10951.7879702E-55.75295351
AGATGAT7300.00540251565.75295351
TGCGGTT7950.00220804345.723592364
Produced by FastQC (version 0.11.5)