FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e2-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e2-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420640
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGAC23950.08.183141
GACTTGA20200.07.79702955
TATGACT23400.07.77723032
GCAACCC7402.1065935E-67.569433747
TGACTTG21800.07.54560574
TGATGTC17050.07.39002949
GCGTAAG6251.1949863E-47.279999710
CAACCCG7754.207963E-67.22758748
CTATATG8251.39043E-67.2116131
CGCAACC12655.2750693E-117.195449446
ATGACTT24950.07.0132873
ATCTCGA5000.00563491466.9995071
CCCGATC8057.4062773E-66.95823651
ATATAAG15251.8189894E-126.88476131
ACCCGAT8854.164607E-66.725057650
GATGTCA16700.06.70658710
GGCGTAA5800.00319558436.63793139
GTCATGT20050.06.632482513
AGGCGTA6400.00123803766.56250058
TGTCATG21200.06.438452212
Produced by FastQC (version 0.11.5)