FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e2-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e2-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511649
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGT950.00721278818.420542
GGTCATC2150.00477629711.39578458
ATATACG6855.7750167E-67.6637671
CCCGATC8755.664515E-87.60079451
CAACCCG7957.7179175E-77.485058348
CCGATCT9503.074274E-87.3691952
TGACTTG20250.07.25905664
GCAACCC8652.920895E-66.87933147
ACCCGAT9401.4950456E-66.702827550
AACCCGA10056.3649895E-76.61760649
TATGACT23400.06.5810132
ATATGAC24750.06.36324831
GTCATGT17853.6379788E-126.274127513
ATGACTT25150.06.2622513
CTATATA24050.06.2574151
TGTCATG17359.094947E-126.253221512
AGAAAGG8451.0837099E-46.2126391
ATCCCTT6200.0064137446.20929861
CTCTTCC8551.2737782E-46.13997651
ATCCATG8551.2737782E-46.13997651
Produced by FastQC (version 0.11.5)