FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e1-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e1-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856670
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGG400.002419408635.00119861
GCGCTCA950.007218654318.41821130
AGTTCCC2600.001597612410.7681481
TGGACTG3300.00106528539.54629653
TTAGCCG3800.00384854978.287971525
CTATTCG4804.2896357E-48.0200281
CTTCACT9552.6370617E-76.96265131
CCGATCT10703.749119E-86.867913721
CCCGATC9753.7868995E-76.81927720
ATCCTCT12301.1466909E-86.54405831
CTATTAG8853.0110858E-56.32704831
ATTATGA23250.06.17142341
ACCCGAT10407.836023E-66.056757519
AGTTGTC7000.00339587365.99939781
AGACTTC7050.0036765215.95684861
CCGCAAC18757.2759576E-115.787845
ATTTAAG17151.6989361E-95.71371171
CAACCCG9253.730146E-45.674800417
ATAAACG8700.00105282155.6316181
TATAAGG7500.00730317975.59943772
Produced by FastQC (version 0.11.5)