FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e1-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e1-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547163
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAGC900.005269780319.44675449
ATATAGG3100.00683320279.0316811
ATCCTTT5251.35129485E-58.6661121
AAGAGCG5653.3943194E-58.05331870
CGCAACC4200.0094151537.4995215
AGAGCGT4200.0094151537.4995262
AAGGATT5350.00136068637.19580133
CTTTAAG5550.00200611146.93649341
ATATAAT15401.8189894E-126.81774571
ATATAGA9854.4706394E-76.75083731
ATCTTCT7954.6422487E-56.60335161
GAAGAGC6904.0000596E-46.594986469
CGGAAGA7004.765306E-46.500772567
TCGGAAG7004.765306E-46.500772566
ATATGAC10004.1032818E-66.2995971
TATAATA11206.1195897E-76.24960042
ATATATG10802.1818705E-66.1570141
TTGCGGT6950.00311881936.04333163
TTGTGTC6950.00312200826.04277917
ATATAAG6950.00312200826.0427791
Produced by FastQC (version 0.11.5)