FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e1-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e1-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181405
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG1708.1836985E-414.41073114
CTCCCGG2100.00400845811.66689956
CGGCTAA5109.078394E-79.60781411
CTTCGGC5351.7210643E-58.5046478
ACTTCGG5855.820795E-68.3762367
GACTTCG5502.1886754E-47.63634066
ATATGAC41100.07.4087371
TATGACT39350.07.2934982
CTATTAC10301.8026185E-87.1359021
TCGGCTA6602.3380894E-46.893919510
TGACTTG36200.06.86462074
GACTTGA31500.06.77775765
AGGGTAT5200.008244436.72997826
GCAACCC13804.110916E-106.59493947
ATGACTT41950.06.59100963
ATCCCTC5900.00382453186.52540451
GATGTCA27700.06.444024610
CTTAGAG8702.3415061E-56.4367631
TGATGTC27350.06.39851869
GGCTTAC6050.00496479036.36434747
Produced by FastQC (version 0.11.5)