FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_UB_e1-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_UB_e1-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263817
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG800.002666688521.87585358
CTCGCTC1400.003753221815.00058666
GAATCGG2000.002782512512.24950927
TACGGAC3257.757892E-510.76965154
CGGACTT3702.5121439E-510.40581156
ACGGACT3501.6432806E-49.99999455
TACGCCT3500.0018236348.99963931
TTACGCC3904.8718543E-48.97399930
ACGCCTT3600.00235558058.74964932
GCAACAC4108.011289E-48.53691947
ATTAACG3900.00485319558.0769191
CGCAACC11353.6379788E-128.01793446
CCGCAAC14450.07.751167345
GCCTAAG17250.07.71044664
ATATGAC22250.07.7078611
TATGACT21450.07.66899352
CAACCCG6007.199669E-57.58392948
TGACTTG19650.07.48091174
GACTTGA16700.07.3353255
TAAGCCT9101.1301745E-77.307687861
Produced by FastQC (version 0.11.5)