FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e3-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e3-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29998
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTGC150.002203749570.0000142
TGATCAT150.002203749570.0000143
TGCTGAT150.002203749570.0000146
AGTGCTG150.002203749570.0000144
GCTGATA150.002203749570.0000147
CCTATAA200.00688644152.53
CTGATAG200.00688644152.548
CGGGTTC200.00688644152.525
CCACTTC200.00688644152.515
TCCATAA200.00688644152.57
GTGCTGA200.00688644152.545
CAATGTA400.002382133435.023
TCAATGT400.002382133435.022
ATCCTAT450.004242439831.1111091
ACTAAAC500.00710054528.00000248
Produced by FastQC (version 0.11.5)