FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e3-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e3-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386828
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG150.002223785370.0000110
GTCCGAC150.002223785370.0000164
GACGGCA207.921978E-570.025
CAGTACG252.3890476E-455.99999627
GGGGATC200.006948567552.570
ACGCCTG200.006948567552.552
ACTGCGC200.006948567552.549
GGCACGG200.006948567552.54
GCAACGG200.006948567552.562
TGGCGGC200.006948567552.521
GGCCCTG200.006948567552.518
GCCTGGC200.006948567552.567
CGCTCAG200.006948567552.524
GAGCGGC200.006948567552.554
GCCTCGC200.006948567552.519
GCGCTCA200.006948567552.523
ACCCCCG200.006948567552.541
TCGGTGA200.006948567552.515
CCCTGGG200.006948567552.539
TGCCCTG352.0848844E-550.028
Produced by FastQC (version 0.11.5)