FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e3-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e3-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271566
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATG150.00222471769.9869231
AGGGTCT150.00222471769.9869229
TGGGCCC150.00222471769.9869229
GTAACGC150.00222471769.9869217
CCTTGCG150.00222471769.9869214
TCGCGAC150.00222471769.9869210
GGCTCAC150.00222471769.9869223
GATAGGC150.00222471769.9869219
GCTCGGC308.353938E-658.34392544
CAGCGGC252.3899796E-455.98954426
AGGACCG200.006941361452.50953369
TGGCCCA200.006941361452.50953342
CCAGTGT200.006941361452.50953351
CCAGTCC200.006941361452.50953346
TAAGCCC200.006941361452.50953368
ACACGGA200.0069464252.49986336
CCGTGGA200.0069464252.4998635
GCACGGG200.0069464252.4998635
CGTTCGG200.0069464252.49986366
AGGCACG200.0069464252.4998633
Produced by FastQC (version 0.11.5)