FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e3-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e3-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246547
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCA150.002222774370.0004048
AGGGCCT150.002222774370.00040466
CCTGCCG150.002222774370.00040457
GACTCGC207.916058E-570.00040448
CCCGGAG252.387272E-456.00032451
GGACGGC252.3896668E-455.98896429
GGCAGCA200.006939860552.5109638
GGCCTCG200.006939860552.5109663
ACGCGGG200.006939860552.5109639
CAACGCG200.006945432652.50030568
GCAACGA200.006945432652.50030568
CCGGAGC200.006945432652.5003054
CCGTGGC200.006945432652.5003057
TCGGGCC200.006945432652.50030531
CCGCTCG200.006945432652.50030570
GACCCAG200.006945432652.50030556
CGGGCCT200.006945432652.50030532
GGGCCTA200.006945432652.50030533
TCGCGCT200.006945432652.50030567
GTTGCCC200.006945432652.50030535
Produced by FastQC (version 0.11.5)