FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e3-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e3-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300233
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATG150.002222333870.0076658
GTCGTTC252.388857E-455.99680337
AGGCGTG200.006944053352.50574558
TTGAGCG200.006944053352.50574544
ACTACCG200.006944053352.50574570
GTACGGC200.006944053352.50574566
GGCAGGT200.0069486352.49699814
CGAACGT200.0069486352.4969986
GACCGCA200.0069486352.49699839
CGCGAGG200.0069486352.49699814
GGCCAGT200.0069486352.49699862
CGGTCCC200.0069486352.49699837
CCGATGG305.8740797E-446.66424
CGCACAC404.5882058E-543.7474986
TGTGCGG350.00125361240.0043846
CGGTAGC350.00125361240.0043850
GCGGTAG400.002415299435.0038349
CGCTCAC400.002415299435.0038356
CATGGGT400.002417274334.99799760
AACGCAC400.002417274334.9979974
Produced by FastQC (version 0.11.5)