FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e2-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e2-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333289
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCC150.0022223870.00943
ATCGGGC150.0022223870.00947
GTGCCGG252.836925E-669.99857
GCCCAGA200.006944193552.50675261
AGCCCGG200.006944193552.50675248
GCGCTGC200.006944193552.50675239
GCCCGGA200.006944193552.50675249
AGCGTAG200.006948316552.49887511
GCAGCGT200.006948316552.4988758
TGCCGGT200.006948316552.4988758
GCGCTTT200.006952441852.49099714
GTGCATC459.1890004E-538.88805455
GTCTCCC400.00241545235.004540
ATTCGCC400.00241545235.004570
TAGGCAG400.00241545235.004559
GAAGGCG400.00241545235.004544
TCGCAGT400.002417231234.9992512
GCCAGAT400.002417231234.999256
AAGCGTC400.002419011434.99425
GGGTCGG450.00430140631.11511239