FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e2-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e2-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214350
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCC150.002222429570.043
CGAGCTC150.002222429570.037
CGATAGG150.002222429570.062
CCCGCCT150.002222429570.035
CTGCCGT252.3866363E-455.99999645
TCGGAGG200.00694436252.548
GCACAGG200.00694436252.554
ACTACGC200.00694436252.515
CCTGCCG200.00694436252.544
CCGCACT200.00694436252.529
AGTCAGC305.868647E-446.66666439
TGGGCAT305.868647E-446.66666434
CTGTGGG459.1768685E-538.8888966
GCACGTG400.002415059635.040
TCCTATG400.002415059635.06
GGGCATG400.002415059635.035
GGAGGCA400.002415059635.039
CCGCTTG552.985395E-431.8181856
CGCTTGT552.985395E-431.8181857
CAGGTAT450.004300721531.11111334