FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e2-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e2-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296831
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCTC150.002223061670.00168632
CGAGTCG150.002224547869.9898929
GCGCCCT150.002224547869.9898925
CCGACCT252.387854E-456.00134710
CAGCCCT200.006946321652.50126312
ACGCACG200.006946321652.50126336
CGCACGG200.006946321652.50126337
CCCGACG200.006946321652.50126362
CGGCGGC200.00695095252.49241630
CGCCTAC200.00695095252.49241625
GCCCTGT200.00695095252.49241614
AGCGCCC200.00695095252.49241624
TCGACCT200.00695095252.49241621
CGGCCCT305.8716204E-446.6677939
TAACGGC305.8716204E-446.6677936
TTGAGGC305.8716204E-446.6677945
CAGGGAT305.8716204E-446.6677939
AACGGCC305.8716204E-446.6677937
GTGCCAC305.8716204E-446.667792
GCGCTCT404.5858797E-543.7510535
Produced by FastQC (version 0.11.5)