FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e2-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e2-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352002
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACG150.002222580470.0082541
TGACGAC150.002222580470.0082547
ATGCCTC252.3873615E-456.0065949
GTCGACC252.3890384E-455.9986437
ACGCGGG252.3907161E-455.9906819
TGCCTCG200.00694481752.5061850
GGCGGGC200.00694481752.5061839
GCAGGGG200.00694481752.5061866
CTGCCCG200.006948720752.4987252
GTAACGC200.006948720752.498722
CCGGGTT200.006952625752.49126421
AGGCGCC200.006952625752.49126411
ACGCTAG305.870406E-446.672165
GGGATGC305.870406E-446.672165
GAGACGC404.585092E-543.7551554
GCGACCT350.001254732439.9990232
CGACCTG350.001254732439.9990233
AGCGAGG350.001255609339.99334724
GCGAGGA400.002419135834.99417525
TGTCGTG652.0982105E-532.31149760
Produced by FastQC (version 0.11.5)