FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e1-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e1-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54256
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAC200.006911845452.50899559
ACTCCTC200.006911845452.50899541
ATTGGGG400.002397204735.00599764
TTGACTA450.004269104531.1164449
AGCTCAA450.00428844631.0877612
Produced by FastQC (version 0.11.5)