FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e1-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e1-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217414
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCCT200.00694810652.49309569
GGAGCAT400.00241396735.0034558
TGGGTAG450.004298778331.11417848
ACACCTT950.00719773218.418633
TAGTTAT1500.005919977613.998163
CCTTTTC2505.2234063E-613.998163
CATTTCA1550.0073555613.5466074
ATCTATG1600.00905915413.12629434
GAAAATC2200.0056398311.13746244
AAGATAA2300.00786179610.6507745
TTAATTG3050.0059463859.18123336
TCCTTTT4251.137991E-49.0576322
ATTAGTA3600.0023413378.7488511
AGAAAGA4158.976036E-48.4326271
GAAAGAA4300.00127592488.1384652
ATATATT8406.636197E-45.83256671
Produced by FastQC (version 0.11.5)