FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e1-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e1-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551157
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGC200.006951194752.4979627
TGCGTGG451.6856884E-646.66061816
GCGTGGA500.00721172527.99637217
CTTAGCA1250.00176148116.7993471
TTCCGAG1450.00473412314.4835098
TCCGAGA1500.00592802214.0007269
GAGGCCA1600.009085095513.12568135
TCTCTCC2408.154321E-411.6672713
CTATAGA3358.339326E-611.492091
TGTCAAG2500.001147789811.20058239
AACTCCA2350.00925105810.42418126
GGATGGA2750.002539218210.18234670
CATAATG2950.0045424089.4911571
ATCACAA4401.6697556E-48.749662
CATAAGA4107.9995167E-48.5362541
ATCACTT4050.00680052167.77747541
CTTAATA5250.00111342847.33304831
ATATATG12604.9112714E-117.2219411
ATTAGTA9552.6105045E-76.963081
ATTCTAA5550.00200564746.9366671
Produced by FastQC (version 0.11.5)