FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_ColSPL9_Col_B_e1-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_ColSPL9_Col_B_e1-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310675
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCT350.001253673540.00441756
GGCACTC400.002417325534.99822655
CTGGAAC450.004301343631.11454654
GCTATCA900.00526500719.4465964
AGGCACT900.00526500719.4465954
AGGAGGA1350.00294112415.55476951
CTTACTA1350.00294112415.55476951
AACGAAC1500.005918035714.00154546
AATGATC2300.00786986610.6516342
ATATAGA4550.0022315557.6919191
ATATGTA7409.3118974E-46.14833741
ATATTAA7600.0083852375.52603631
ATAATTA8500.0048513075.35267071
ATTTATT10000.00105066825.24973441
Produced by FastQC (version 0.11.5)