FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_C24SPL9_C24_B_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_C24SPL9_C24_B_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34777
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCAGGGCGGCGGGCACGGCGACCGGCCGATCGTGGTCTCCTACGACTC380.10926761940362885No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGC150.00220674370.049
TTAGGTG150.00220674370.023
TACCCAA150.00220674370.052
ATAGGTA150.00220674370.017
GCCTCGT150.00220674370.03
CTCATAG150.00220674370.027
ATCCACA150.00220674370.015
GAAAAGC150.00220674370.062
CAGCTGT150.00220674370.039
TCATAGG150.00220674370.015
TCATACC150.00220674370.015
TCTATTA150.00220674370.047
AGGTATT200.00689572252.519
GTATTAT200.00689572252.521
CAAGTAG200.00689572252.57
TCCGAGG200.00689572252.520
GCGAACC200.00689572252.550
TTAGAGG200.00689572252.531
AGAAGTA200.00689572252.515
GGTCCTA200.00689572252.54