FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_C24SPL9_C24_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_C24SPL9_C24_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817652
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGG1100.063.6416445
GGCATAC650.059.221229
GGGGCGA653.6379788E-1253.8506242
CGCGGCC608.367351E-1152.504364
CGGGCAC1350.051.8466412
GGGCCCT750.051.33759768
GGGCCCA900.050.5597532
ACCGGCC1800.050.54738623
GCGCACT707.2759576E-1250.0010969
GGCCCGC352.08894E-549.99191717
GAGGGCG506.1945684E-849.00406665
GCGGGCA1450.048.27396411
CGGGGCA800.048.1319448
GACCGCG451.6835183E-646.67339747
CGCGCAG305.8759964E-446.67054468
GGCCCTC751.4551915E-1146.6705464
TTACGCG751.4551915E-1146.6705468
GTTCGGC751.4551915E-1146.667691
CTCGCGA1350.046.65912615
GGCGACC1500.046.65912619
Produced by FastQC (version 0.11.5)