FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_C24SPL9_C24_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_C24SPL9_C24_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837468
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT1050.066.66427669
GGCTGGC352.9732837E-760.00501641
TCGGGCC509.822543E-1055.9979934
GGCCCTC700.054.9980364
ATGGCGC653.6379788E-1253.8442272
GGCACGG850.053.52749614
TGCGCAC1350.051.8499968
CGGGCGC552.2864697E-950.9072722
AGGCGTG506.19475E-849.00409743
CGCGGCC506.200571E-848.9982454
GGCATAC1000.048.99824529
TGAGGGG506.200571E-848.99824570
CGCGCAT800.048.13189752
GCGCCCC800.048.12327664
AGCGCGC305.8743317E-446.67335554
CGCCCGC1050.046.67335553
CCCCGTT451.6856575E-646.66499760
GGGCAGG900.046.66499710
GGCGCAC451.6856575E-646.66499730
GTTCGGC751.4551915E-1146.6649931
Produced by FastQC (version 0.11.5)