FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_C24SPL9_C24_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_C24SPL9_C24_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818623
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGC401.0657459E-861.2563154
GGGCCCT401.0662916E-861.25256768
AGTCGCG200.00694849552.5054135
GTGCGCT407.4858326E-752.49578529
CGGGCCA200.00695521352.49257714
CCTGGGG200.00695521352.49257725
CGCAACG200.006956893552.48937224
GTCGCGC352.0871825E-549.9990434
CGGGGCC352.0871825E-549.9990458
CCGGGTT800.048.12113628
GTGCCGA604.9276423E-946.67717740
CGCGACT451.6825816E-646.67717441
GCCCCCC305.877192E-446.6686268
GCGGTCT604.9367372E-946.6686268
CGCGTCT305.878962E-446.66576831
CGCGGCC451.6861268E-646.662924
TGCGGCC451.6861268E-646.6629211
CCGCAGC703.8380676E-1045.00463545
AGGGCGG703.8562575E-1044.9963875
TAAGGGG551.3096724E-744.5554936
Produced by FastQC (version 0.11.5)