FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38340262
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA622050.063.455816
GCGCTAG308850.063.291625
GGGTCCT357800.062.7871323
GGCGCTA331150.062.2297064
GGTCCTA419600.061.3440554
CCGCTAG283400.061.160545
GCCGCTA285100.059.997074
GAGCGCT233000.059.26793
GTCCTAT429700.057.9409685
GGGCGCT186450.057.1578453
CGGCGCT247750.056.8408473
GCGCTAT156050.055.4915165
AGCGCTA165250.055.2805824
CGGTCCT186850.054.751073
GCGCGCT184850.054.5674323
GCTAGAT639100.054.3639037
AGCGCTC201300.053.9665954
GCGCTCA158050.053.925975
GTCCTAG273600.052.7884255
GTGTCCT284650.052.287113
Produced by FastQC (version 0.11.5)