FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65494
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGATC500.070.000011
AGCGTCC252.8111517E-670.000012
GCTGGTC252.8111517E-670.000011
CCTACGC252.8111517E-670.000017
CCTACGA252.8111517E-670.000017
CCTACCG252.8111517E-670.000017
TGAGTCC252.8111517E-670.000012
CCTGTAC252.8111517E-670.000017
CCTAACT252.8111517E-670.000017
TGCATCC252.8111517E-670.000012
CGGGCTC252.8111517E-670.000011
CCTGGGT252.8111517E-670.000017
GTGGTCC950.070.000012
GCGTATC252.8111517E-670.000011
AGTGTCC500.070.000012
ATCCTGG207.872973E-570.05
CTAGAGG150.00221551670.08
GGAATCC401.3278623E-1070.02
CACGGTC150.00221551670.01
CTTCCCG150.00221551670.068
Produced by FastQC (version 0.11.5)