FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261631
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCC700.070.000012
CCTGTCG301.02119884E-770.07
CTCGCGT207.917192E-570.011
CGGTCCT4750.070.03
GCGGTCC1800.069.999992
GGTCCTC5850.068.803424
GTCCTAT12950.068.378385
GGGGTCC3500.068.02
GGTCCTA13050.067.854414
GGGTCCT10700.067.710283
AGGGTCC3050.067.704922
AGGTCCT6000.067.6666643
CGTTCCT4450.067.640453
GTGTCCT7400.067.635133
GTCCTAA11250.067.5111165
GGTCCTG4150.067.469884
TGTCCTA8250.067.4545444
GCGGGTC1300.067.307691
CTGTCCT3850.067.272733
GTTCCTA10900.067.1100854
Produced by FastQC (version 0.11.5)