FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100930
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGTCCTGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGA1290.1278113544040424TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTA1900.070.000015
GTCCTCG650.070.000015
CGGTCCT2600.070.000013
GCGACTC150.002219010870.01
GCGCGTC150.002219010870.01
CGGCATC252.822242E-670.01
CGCGCTC252.822242E-670.01
GCAGTCC500.070.02
CCTACTG401.3278623E-1070.07
GTGATCC1000.070.02
GTGGCTC207.893672E-570.01
ACGGGTC150.002219010870.01
CCTCGCA252.822242E-670.07
GCTCTCC301.0148324E-770.02
GGGCGTC301.0148324E-770.01
CCTCAGA252.822242E-670.07
GCGATCC500.070.02
GGTTGTC353.6689016E-970.01
CAGTCCT1100.070.03
GCTGTCC800.070.02
Produced by FastQC (version 0.11.5)