FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534038
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT24150.068.846473
GGTCCTA29400.068.57734
GTCCTAT30300.068.042135
AGGGTCC6400.067.8183062
CGGTCCT9500.067.795273
GGCTCCT12600.067.505783
GGTGTCC6600.067.3542562
GTCCTAG14800.067.167915
GGTCCTG7350.067.1486054
GCGGTCC4500.066.8946152
AGGTCCT13450.066.883053
GGCGTCC4400.066.82392
GGGCTCC6200.066.618612
GGGGTCC8050.066.5274352
GGGGATC4000.066.505691
GCGTCCT7750.066.3927843
TGTCCTA18400.066.3915564
GTTCCTA19700.066.2747044
CGTCCTA10000.066.155664
GTCCTAC9800.066.077095
Produced by FastQC (version 0.11.5)