FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370199
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTC1350.070.01
CGGTCCT8500.068.352943
GTTCCTA15200.068.15794
GGTCCTA19900.067.889444
GGGCTCC4850.067.835052
GTGTCCT10450.067.320583
GTCCTAT18050.067.0914155
GGGTCCT16050.066.947043
GCGGTCC4500.066.888892
TGTCCTA12900.066.744194
GTCCTAC7400.066.6891865
GGGTATC2050.066.5853651
GCTCCTA11150.066.547094
GGCTCCT9050.066.519333
GGTCCTT6100.065.9836044
GGGGATC4350.065.977011
GGTCCTC10300.065.922334
TCCTATG12850.065.91446
GTCCTAA15350.065.895775
GGGGTCC5700.065.701752
Produced by FastQC (version 0.11.5)