FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118014
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA109550.068.502594
GGGTCCT92650.068.493123
GTCCTAT113500.067.814715
CGGTCCT40150.067.214573
GTCCTAA85200.067.210685
GGTCCTC54400.066.787164
GTGTCCT63450.066.639213
GGCTCCT44950.066.4222953
AGTCCTA71550.066.335294
GTCCTAG56200.066.142825
CGCTCCT23450.066.123443
GGGGTCC30800.066.0267642
AGGTCCT52800.066.026763
GGTTCCT65950.066.023743
TGTCCTA77300.066.0195544
GCGGTCC19850.065.9486162
GTTCCTA77550.065.896994
AGGGTCC26050.065.704592
GGCGTCC13350.065.547072
GTCCTAC38450.065.543665
Produced by FastQC (version 0.11.5)