FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914534
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT40900.068.983
GGGGTCC12150.068.854622
GGTCCTA47900.068.399324
GTCCTAT49150.068.1553345
CGGTCCT19050.067.434573
GTCCTAA36650.067.141785
AGTCCTA31350.066.992354
GTCCTAG25400.066.8373955
AGGGTCC10700.066.408512
CGTCCTA16050.066.299464
GCGTCCT13950.066.243183
GCGGTCC9800.066.078032
GGCTCCT21650.065.965023
GTCCTCA21050.065.849815
TGTCCTA32200.065.767454
GGTCCTT19550.065.70994
GGTCCTC24400.065.559844
GTTCCTA35000.065.5065464
AGGTCCT21700.065.490423
TCCTATA47400.065.428486
Produced by FastQC (version 0.11.5)