FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011C_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10493616
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT416300.068.6057743
GGTCCTA496050.068.456194
CGGTCCT181750.068.037153
GTCCTAT549550.067.874195
GCGGTCC85600.067.3436662
GGGGTCC139950.067.27542
GCGTCCT137300.066.7129443
GGTCCTC254500.066.549494
AGGTCCT250950.066.528563
GGCTCCT211050.066.502193
GTGTCCT323950.066.479213
GTCCTAA390350.066.432765
GTCCTAG255950.066.350255
CGTCCTA170300.066.34314
GTTCCTA383550.066.095734
TGTCCTA374600.065.974384
GGTCCTG151050.065.8073654
GCTCCTA255850.065.7742544
GGTTCCT319300.065.770213
GGTCCTT213150.065.7484744
Produced by FastQC (version 0.11.5)