FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011B_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011B_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162230
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA150.002221451170.000014
CGGTCCT650.064.615393
GGTCCTA1700.063.823534
TCCTATG850.061.7647066
GTCCTAT1600.061.2500045
CGATCCT352.9599505E-760.03
GTCCTAG900.058.333335
GGGTTCC900.058.333332
GGTTCCT1650.057.2727243
GCGCTCC252.3848985E-455.9999962
GTGTCCT950.055.263163
GTTCCTA1350.054.4444474
GCGGTCC452.6842827E-854.4444432
CGTCCTA452.6842827E-854.4444434
GGGTCCT1550.054.1935463
GGTCCTT850.053.529414
GTCCTTG407.444196E-752.5000045
CGCTCAC200.0069413352.5000046
GGCGGTC407.444196E-752.5000041
GGCATCC407.444196E-752.5000042
Produced by FastQC (version 0.11.5)