FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColTGA5_pool19011B_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColTGA5_pool19011B_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4312941
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT29000.063.242723
GGTCCTA35150.058.9486244
GTCCTAT38200.057.540495
GGTCCTC19800.055.1526874
GGGCGCT3050.052.7880063
GTCCTAG20700.051.9093135
AGGTCCT19850.051.6635673
CGGTCCT13000.050.616463
GTGTCCT23700.050.2120363
GGCTCCT18300.050.110353
GAGTCCT23550.049.9373633
AGGGTCC12600.049.445492
GGGGTCC10450.048.900562
TGTCCTA31100.048.2807664
AGTCCTA28750.047.8444944
GGTTCCT27000.047.575083
GTCCTAA32800.047.2723435
CGTCCTA11800.047.1620144
GGTCCTT19200.047.0322464
GTCCTCT25600.046.622085
Produced by FastQC (version 0.11.5)