FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14742
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAC150.00218134870.000016
TGGTCCT150.00218134870.000013
TCCTTAT150.00218134870.000016
GAGCGCT309.557152E-870.000013
CTATATA150.00218134870.000018
AACCGCT150.00218134870.000013
TTCGCTC150.00218134870.000014
GCTAGAA150.00218134870.000017
TCCTAAT150.00218134870.000016
GCCTAAT150.00218134870.000013
CAGTAAT150.00218134870.000013
CGCTCTA150.00218134870.000016
CCGCTGA150.00218134870.000015
CCGCTAT150.00218134870.000015
CCGCTAG309.557152E-870.000015
GATAATC150.00218134870.000014
AGGCGCT150.00218134870.000013
CCCGCTG150.00218134870.000014
GCTATAA150.00218134870.000017
TTCCTAT150.00218134870.000015
Produced by FastQC (version 0.11.5)