FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378233
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGCT1400.070.03
CCTGCCT150.002223747370.07
CCGCTAG6650.068.421065
AGGCGCT1900.068.15793
GCCGCTA7400.068.108114
CGCTAGA13750.067.709086
CGGTCCT1500.067.6666643
GCGCTAT4150.067.469885
GCGCTAG5500.067.4545445
CGCTAAG2200.066.818186
GACGCTA3250.066.769234
CGCTACT2150.066.744196
CGCTATA4600.066.195656
AGCGCTA5050.065.841584
AAGCGCT2400.065.6253
CGCCGCT3200.065.6253
GCGCGCT3800.065.394743
ACGCTAG2950.065.254245
TCCGCTA2200.065.227274
GGCGCTA6500.065.153854
Produced by FastQC (version 0.11.5)