FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213822
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCC850.070.000012
GCCCCGC301.02030754E-770.02
AGCCGTA150.002222421370.01
GTACCGC207.9138874E-570.02
TGCCCCG150.002222421370.01
TCCTGGG207.9138874E-570.06
GGCACGC150.002222421370.02
CTGAGCG252.833087E-670.01
CACGCTC401.3460522E-1070.04
GTCTCGC150.002222421370.02
AGGCGTC207.9138874E-570.01
CCGCTTA353.6943675E-970.05
TAACGCT401.3460522E-1070.03
CCGTCCT207.9138874E-570.03
CAGGGTC150.002222421370.01
CGCCTAA207.9138874E-570.02
CCCGTGC150.002222421370.045
CGCTAGC1100.070.06
CGCTACC207.9138874E-570.06
CAGGCGC252.833087E-670.02
Produced by FastQC (version 0.11.5)