FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47719
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGC150.00221181870.000012
CTCACTA150.00221181870.000018
TGGAGCG150.00221181870.000011
AGTCCGC150.00221181870.000012
TCCGCTC301.00337274E-770.000014
TCACCGC150.00221181870.000012
GATCGCT207.851091E-570.000013
GGGCTAA150.00221181870.000012
TTAGCCG150.00221181870.000011
GGGTCCT207.851091E-570.000013
GCGCGCT207.851091E-570.000013
GTCCTGT150.00221181870.000015
TTGCTCC150.00221181870.000012
GTCCTCT401.3096724E-1070.000015
GTCCTAG401.3096724E-1070.000015
CTATACT150.00221181870.000018
CTGAGCG150.00221181870.000011
GACCGCT207.851091E-570.000013
TGGCGCT150.00221181870.000013
CTCCCGC150.00221181870.000012
Produced by FastQC (version 0.11.5)