FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36530
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCT550.070.000013
TCGCTAG550.070.000015
GCATAAT309.967516E-870.03
ATCCTAT252.7862352E-670.05
AGCGTAA150.002207638570.02
CGGCGCT309.967516E-870.03
TCCGCTT150.002207638570.04
GATCGCT252.7862352E-670.03
GGGCTCC150.002207638570.02
GGTCGCT150.002207638570.03
GGGTCCT401.2914825E-1070.03
TCCTGCT150.002207638570.06
ACCGCTA252.7862352E-670.04
CCGGCGC150.002207638570.02
GTCCTGT207.8263845E-570.05
GTCCTGG150.002207638570.05
GTCCTGA207.8263845E-570.05
GTCCTCG150.002207638570.05
GTCCTCC207.8263845E-570.05
TGGGTAA207.8263845E-570.02
Produced by FastQC (version 0.11.5)