FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062130
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA62600.067.204426
GCGCTAG23600.067.0338445
CGCTAGT12900.066.4728246
GCCGCTA34650.066.262584
CCGCTAG33350.066.221855
GGCGCTA31250.065.967964
GCGCTCA20800.065.120155
ACGCTAG12850.065.097235
GCGCTAT24250.065.092745
GGGCGCT22450.064.699293
GGGTCCT16650.064.534493
GAGCGCT32450.064.499183
CCGCTAT19800.063.9898535
CGCTATA26150.063.843176
AGCGCTC27400.063.7408334
GGACGCT10500.063.6666223
AGCGCTA23200.063.6637464
CGCTCTA15550.063.4726266
CGCTCAT17500.063.399966
GGCGCTC17300.063.3236544
Produced by FastQC (version 0.11.5)