FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587568
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA52350.067.260716
CCGCTAG25700.066.7333765
GCCGCTA26850.066.6126564
AGCGCTC23950.066.3484044
GCGCTCA16450.066.172065
GGCGCTA22650.065.829654
CAGCGCT11000.065.547293
GAGCGCT30900.065.131273
GGGTCCT13400.065.039123
GGGCGCT18050.064.9602663
GTCGCTA16650.064.956774
GTTCGCT12000.064.751813
GCGCTAT19550.064.630965
GCGCTAG18750.064.588465
CGCTAGT9000.064.557366
CGCTAAG8450.064.202976
CGCTATA20500.064.1969156
CGCTCAT11400.064.168466
TCGCTAG16100.064.1322255
GGACGCT6550.064.123933
Produced by FastQC (version 0.11.5)