FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580801
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT3250.067.846153
GGTCCTA5150.066.601944
GGGTCCT5650.066.283193
CGCTAGA14800.066.216226
CCGCTAG7100.066.056345
CCCGCTA3550.066.056344
CGCTCAA5800.065.775866
GCCGCTA7250.065.6551744
GCGCTAG6400.065.6255
GCGCTAT6900.065.4347845
GCGCTCA5150.065.2427145
GGGCGCT5650.065.044253
GCCCGCT2750.064.9090963
GGCGCTA7700.064.5454564
CGCTAGT3650.064.2465746
GTCGCTA5300.064.05664
AGCGCTC7000.064.04
GGTCGCT2900.063.9655153
GCGCTGC1150.063.9130485
AGCGCTA6750.063.7777824
Produced by FastQC (version 0.11.5)