FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_input_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743921
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT3900.067.307693
GGGTCCT6100.067.131153
CCGCTAG10550.067.014225
CGCTAGA20750.066.457836
GCCGCTA10750.066.41864
CGCTCTA5350.066.074766
GCGCTAG7950.066.0377355
GGCGCTA8450.065.857994
CGGCGCT3800.065.394743
GGGCGCT5800.065.172423
GTCCTGT5650.065.044255
GAGCGCT11800.064.957633
CGCCGCT4750.064.84213
GCGCTAT8050.064.782615
GTCCTAT6350.064.488195
GCGCTCA6900.064.420295
GCGCTCT6150.064.3089455
GTCGCTA7300.064.2465744
GTCGCTC3950.063.7974624
CGCTATA9800.063.5714236
Produced by FastQC (version 0.11.5)