FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7358
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA150.002137746770.000014
GCTAGAA150.002137746770.000017
GTGTAAT150.002137746770.000013
CGCTCTC150.002137746770.000016
TCGCTTT150.002137746770.000015
TAATCCC150.002137746770.000016
GCCGCTC150.002137746770.000014
GCTCCTG150.002137746770.000014
TTAATCC150.002137746770.000015
GTAATAC150.002137746770.000015
TTTGTAA150.002137746770.000012
CCGCTCT207.417047E-570.05
CGCTAGA207.417047E-570.06
GGATAAT207.417047E-570.03
TAATCCT207.417047E-570.06
ACCTAAT207.417047E-570.03
TGTAATA207.417047E-570.04
GTTCGCT252.5700774E-670.03
TAATAGA252.2376512E-455.9999966
TGTCCTC200.006681737552.54
Produced by FastQC (version 0.11.5)