FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140651
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA1750.070.000014
TACGCTG252.8280429E-670.04
AGTGCGC301.01776095E-770.02
CCGCTAG1650.067.8787845
GTAATAC1600.067.812495
TAATACC1200.067.083336
CGCTAAA1850.066.216226
CCGCTAT2400.065.6255
TACCGCT750.065.3333363
TAATGTG1300.064.615396
GGTAATG1750.064.000014
AGGTAAT2750.063.6363643
GGGCGTA550.063.636361
GGGCGCT2150.063.4883733
GCATAAT1600.063.4374963
GGTTAAT1600.063.4374963
GACCGCT1050.063.3333363
CGCTAGC1050.063.3333366
TAATCTG1550.063.225816
GCGCTGA950.062.6315845
Produced by FastQC (version 0.11.5)