FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48510
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTA650.070.000014
AGTCCTG207.8524215E-570.04
GGAGCGC150.002212043470.02
TCCGCTC252.8001523E-670.04
GGTAATG600.070.04
CGTATCC150.002212043470.02
TTAATCA401.3096724E-1070.05
GGGCTAA252.8001523E-670.02
TCCTGTA207.8524215E-570.06
ACCGCTA301.00373654E-770.04
GTCCTAA150.002212043470.05
TAGAAAC207.8524215E-570.09
ATAGCAG150.002212043470.08
ATTCGCT401.3096724E-1070.03
GGGAGCG150.002212043470.01
TCCTATC252.8001523E-670.06
CCGCTAC353.614332E-970.05
TCCTAAG150.002212043470.09
ATATCGT150.002212043470.08
TGCGCTA252.8001523E-670.04
Produced by FastQC (version 0.11.5)