FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27243
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGTT150.00220156170.08
TAATAGC150.00220156170.06
TAATACC309.8716555E-870.06
TAATAAC207.7905E-570.06
TAATAAA500.070.06
TCCGCTA353.5361154E-970.04
GGTAATG150.00220156170.04
TTAATCG150.00220156170.05
TTAATAC150.00220156170.05
TACTTCC150.00220156170.09
CGACGCT207.7905E-570.03
CCGATAA150.00220156170.02
ATAATGC353.5361154E-970.05
ATAATAC309.8716555E-870.05
GCTATGT150.00220156170.07
ATTAATA150.00220156170.04
TTGTAAT252.7670867E-670.03
AGGGTAA252.7670867E-670.02
CGCTGTG150.00220156170.06
CGCTGTA150.00220156170.06
Produced by FastQC (version 0.11.5)