FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14419
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTAA150.002180434870.02
GAGCGCT252.7010483E-670.03
TTCGCTC150.002180434870.04
TTCGCTA401.200533E-1070.04
TGCGCTA252.7010483E-670.04
TCCTATA150.002180434870.06
AATCACT150.002180434870.07
GCCTAAT207.6661956E-570.03
AGCTAAT150.002180434870.03
CCGCTCT150.002180434870.05
CGCTCGA150.002180434870.06
GATAATG150.002180434870.04
AATTATC150.002180434870.07
CGCTCAT401.200533E-1070.06
CGCTTAT150.002180434870.06
AGGGCGC252.7010483E-670.02
TAATGTC150.002180434870.06
TAATGAT353.3796823E-970.06
CGCTATT252.7010483E-670.06
CGCTATG150.002180434870.06
Produced by FastQC (version 0.11.5)